The sensing probes are coated with an oxygen-sensitive material. When optically excited, this material fluoresces and emits light depending on the amount of oxygen present. The processes the emitted light signal into an oxygen concentration measurement.

The LEDs of the device emit blue light with a wavelength range of 435-485 nm.

No, accommodates normal cell growth workflow, i.e. same seeding density, media, treatment, etc. No special assay is required for an experiment using . After the experiment, you can collect and utilize the cells in the well plate.

Yes, the streams your data in real-time for you to access from anywhere!

is connected to the Hub via a USB-C cable. This wired connection design ensures there is no power interruption to the during the experiment. The USB-C cable can be inserted through a port at the back of an incubator (if available).

Oxygen concentration (O₂C) readings are available immediately after the experiment begins; oxygen consumption rate (OCR) is shown 30-60 minutes after the beginning of each experiment. This time period allows oxygen gradients to form in each well.

Since oxygen measurement is temperature-dependent, expect reliable data to be available 30-60 minutes after the initial setup. The goal is to allow time for the incubator to equilibrate back to its normal environment, i.e. 37 C, 5% CO₂, etc. This initial wait period also allows oxygen gradients to form in each well.

works best at an oxygen level of 0-20%. If your application requires a higher oxygen level, we will be happy to discuss further.

Our customers usually run experiments with a duration of 3-7 days. The optimal duration ranges from 1 day to 14 days. There are also experiments as short as 12 hours and as long as 21 days.

It takes less than 10 minutes to set up an experiment in our software.

You can seed the cells like you normally do. allows you to follow your typical cell growth workflow.

Non-adherent cells that settle to the bottom of the wells also work well with .

No, accommodates normal cell growth workflow, i.e. same seeding density, media, treatment, etc. No special assay is required for an experiment using .

Simply separate the device from the sensing lid; transfer the well plate and sensing lid into a biosafety cabinet and treat the cells as normal in a sterile environment. Once treated, place the sensing lid back onto the well plate, transfer it back into the incubator and reconnect the lid with the device.

The was originally designed to accommodate Falcon and Nunc 96 well plates with the following catalog numbers. In addition, we have validated a lot of other 96 well plate types, please reach out to our team to share your desired plate model and we will help you identify the correct probe lengths to accommodate your cell model system.

Nunc (167008) & Falcon (353072)

Yes - our current software allows users to download data in JSON or CSV formats.

You can purchase lids here.